CRISPR-Cas9 gRNA Specificity Analyzer

A functional Python bioinformatics tool for analyzing and ranking CRISPR guide RNAs (gRNAs) based on off-target specificity using NCBI BLAST

This project was created to help students evaluate the specificity of CRISPR-Cas9 gRNAs by automating off-target analysis through BLAST, then scoring and ranking the results with customizable logic.

How to run gRNAlytics

1. Clone this repo:

• git clone https://github.com/MisplacedOrange/gRNAlytics
• cd gRNAlytics

2. Install dependencies:

• pip install -r requirements.txt
• Make sure AchillesCommonEssentialControls.csv is in the same folder.

3. Run the code!